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Sun JH, Huang M, Fang Z, et al. Nerve bundle formation during the promotion of peripheral nerve regeneration: collagen VI-neural cell adhesion molecule 1 interaction[J]. Neural Regeneration Research. 2022, 17(5):1023-1033.
Journal Neural Regeneration Research IF(2021) 5.135
doi 10.4103/1673-5374.324861. Product NCAM120
Services Recombinant Protein Production System Mammalian Expression
The DNA segment encoding the extracellular region of Ig-like domains (Leu20 to Val495) and that encoding the extracellular region of FnIII domains (Gln496 to Gly740) of human NCAM120 were inserted between the BglII and XhoI sites of the mammalian expression system vector proEM™ (DetaiBio, Nanjing, China)
Guo XF, Zhang YY, Kang J, et al. A bispecific decoy receptor VEGFR-EGFR/Fc binding EGF-like ligands and VEGF shows potent antitumor efficacy[J]. Journal of Drug Targeting, 2022, 30(3):302-312.
Journal Journal of Drug Targeting IF(2021) 5.121
doi 10.1080/1061186X.2021.1961791 Product VEGFR-EGFR/Fc
Services Recombinant Protein Production System Mammalian Expression
After codon optimization, the full gene coding for VEGFR-EGFR/Fc was synthesized by DetaiBio company (Nanjing, China), then was inserted into the expression vector proEM.
Luo ZH, Liao T, Zhang YN, et al. Ex vivo anchored PD‐L1 functionally prevent in vivo renal allograft rejection[J]. Bioengineering & Translational Medicine. 2022
Journal Bioengineering & Translational Medicine IF(2021) 10.711
doi 10.1002/btm2.10316 Product PD-L1 + APT542
Services Recombinant Protein Production System Mammalian Expression
The protein amino acid sequence of rat-derived PD-LI and anchoring structure APT542 were codon optimized using MaxCodonTM Optimization Program (V13) (DetaiBio). The PD-L1 + APT542 gene was inserted into the expression vector pcDNA3.1(-) by wholegenome synthesis and double enzyme digestion. Accuracy of the final expression vector was confirmed by restriction enzyme digestion and sequencing. Finally, the vector was transformed into DH5a cells, and the plasmid was extracted using a plasmid extraction kit. The plasmid was then transfected into mammalian HEK293 cells for transient expression, and the fusion protein map-PD-L1 was purified using affinity chromatography.
Huang Y, Yu SH, Zhen WX, et al. Tanshinone I, a new EZH2 inhibitor restricts normal and malignant hematopoiesis through upregulation of MMP9 and ABCG2[J]. Theranostics, 2021; 11(14):6891-6904.
Journal Theranostics IF(2021) 11.556
doi 10.7150/thno.53170 Product EZH2&SUZ12
Services Recombinant Protein Production
Zebrafish EZH2(Detai Bio) / SUZ12(Detai Bio)
Xu WF, Che Y, Zhang Q, et al. Apaf-1 Pyroptosome Senses Mitochondrial Permeability Transition[J]. Cell metabolism. 2021, 33(2):424-436.
Journal Cell metabolism IF(2021) 27.287
doi 10.1016/j.cmet.2020.11.018 Product CASPASE-4
Services Recombinant Protein Production System Mammalian Expression
Human CASPASE-4-pcDNA3.1(-)-AMP-His(Detai Bio)
Human CASPASE-4-pcDNA3.1(-)-AMP-HA(Detai Bio)
Human CASPASE-4(1-90AA)-pcDNA3.1(-)-AMP-His(Detai Bio)
Recombinant pro-caspase-4-eGFP-His(Detai Bio)
Recombinant pro-caspase-11-eGFP-His(Detai Bio)
Recombinant caspase-4 (CARD)-Fc-HA(Detai Bio)
Xu WF, Zhang Q, Ding CJ, et al. Gasdermin E-derived caspase-3 inhibitors effectively protect mice from acute hepatic failure[J]. Acta Pharmacologica Sinica. 2021(42), 68-76.
Journal Acta Pharmacologica Sinica IF(2021) 6.15
doi 10.1038/s41401-020-0434-2 Product caspase 4
Services Recombinant Protein Production System Mammalian Expression
active caspase-4-GFP was ordered from DeTai Bio
Xia AH, Li X, Quan JJ, et al. Mycobacterium tuberculosis Rv0927c Inhibits NF-κB Pathway by Downregulating the Phosphorylation Level of IκBα and Enhances Mycobacterial Survival[J]. Frontiers in immunology, 2021(12):721370.
Journal Frontiers in immunology IF(2021) 7.561
doi 10.3389/fimmu.2021.721370 Product Rv0927c
Services Recombinant Protein Production System Bacterial Expression
Rv0927c gene was synthesized and ligated into pET30a(+) expression vector (DetaiBio, Nanjing, China).
Xu S, Jing M, Kong DM, et al. Chitin binding protein from the kuruma shrimp Marsupenaeus japonicus facilitates the clearance of Vibrio anguillarum[J]. Developmental & Comparative Immunology, 2021(117), 103981.
Journal Developmental & Comparative Immunology IF(2021) 3.636
doi 10.1016/j.dci.2020.103981
Services Peptide Synthesis
A peptide synthesis service was offered by biology company DETAIBIO (Nanjing China),
Sun BQ, Xue YF., Du XL, et al. Identification of genetic determinants of hemolytic activity of Riemerella anatipestifer using random transposon mutagenesis[J]. Veterinary research, 2021(52), 19.
Journal Veterinary research IF(2021) 3.699
doi 10.1186/s13567-021-00900-6
Services Protein purification
The soluble recombinant proteins rRiean_0317, rRiean_0653, and rRiean_1143 were purified by Ni-iminodiacetic acid affinity chromatography (Detai Bio-Tech (Nanjing) Co., Ltd., Nanjing, China)
Li X, Bai LJ, Zhang YH, et al. Novel GLP-1/anti-apolipoprotein B bifunctional fusion protein alleviates diabetes and diabetic complications in combination with low-intensity ultrasound[J]. Life Sciences, 2021(278):119549.
Journal Life Sciences IF(2021) 5.037
doi 10.1016/j.lfs.2021.119549
Services Recombinant Protein Production System Mammalian Expression
All bifunctional fusion proteins were entrusted to Nanjing Detai Bio Co., Ltd. for gene synthesis and recombinant protein expression with purity higher than 98%.
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